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Superoxide development try sensed inside blood and BALF using the CMH spin probe. Blood otherwise BALF products had been accumulated of PBS- and Bleo-treated rats and you will incubated instantly with CMH. The new trials have been relocated to the newest PTFE tubing and you can thumb frozen, and you may EPR analysis is actually accumulated during the 77 K. The newest concentration of nitroxide (CM.) collected in the bloodstream incubated which have CMH (0.dos mM) from the 37 degrees to have ten minute (Profile 4A).
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